Topflash renilla
Web30. apr 2004 · b, Determination of whether inhibition of endogenous LRP6 inhibits Wnt3a activation of the Wnt/β-catenin signaling pathway. 293T cells were co-transfected with the vectors shown, Super(8X)Topflash (to measure Wnt/β-catenin-signaling), and a constitutive expression vector for Renilla luciferase. 24 hours after transfection the cells were ... WebThe protocol I follow is as follows: Day 1 : See 0.2e6 HEK293 Top Flash cells (stable cell lines with Renilla/Luc reporters) in 250uL DMEM-10% FBS, on a 24-well plate. Day 2 : …
Topflash renilla
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Web报告基因是一类使表达产物易于被检测的基因，其作用原理是将报告基因的编码序列及其表达调控序列与目的基因融合形成嵌合基因，在调控序列的作用下进行表达，通过对报告基因表达产物的检测来评价目的基因的表达状况。 常见的报告基因包括萤光素酶（Luciferase）、氯霉素乙酰转移酶（CAT ... WebFrom that 50ng DNA, 15ng are TOPFlash plasmid (Firefly), 10 ng Renilla, and the rest empty plasmid (PCS2 or pCDNA3). Treat the cells O/N with concentrations between 1-3uM of …
Web22. mar 2024 · To perform the high-throughput luciferase screening, the HEK293 cells were seeded in 96-well plates at the density of 5000 cells/well. After 80% confluency, the cells were co-transfected with TOPFlash or NF-κB RE … WebLenti-AP1-TA-Luc-EF1α-mCherry (10^8TU/ml, 报告基因慢病毒)，即Lenti-AP1-TA-Luc-EF1α-mCherry-T2A-Puro，是碧云天自行研发的一种可以在大多数哺乳动物细胞(包括原代细胞和干细胞)中表达AP1报告基因并组成性表达mCherry和
WebA . Oxy186 and Oxy210 inhibit WNT reporter (TOPflash) luciferase activity in A549 cells. A549 cells transfected with TOPflash and pRL-TK were treated with the indicated compounds in the absence or presence of WNT CM. After 24 h, Firefly luciferase activity was measured and normalized to Renilla luciferase activity. WebTop 4 Icons Finish for Team Flash Wild Rift Challenge the World! Team Flash Wild Rift defies the odds to achieve a Top 4 placement at the Wild Rift Icons Global Championship!
WebFirefly and Renilla luciferase are less-than-ideal protein tags due to their large size. NanoLuc® Luciferase is small, bright, and a secreted version of the protein is available - these attributes have made it popular with scientists since its release.
Web今天小编主要为大家简述一下荧光素酶报告基因系统，荧光素酶的种类很多，其中应用较为广泛的是萤火虫荧光素酶（Firefly luciferase，FLUC）和海肾荧光素酶（Renilla … chemistry 8 inclusionWebDescription. Configurations available with the synthetic firefly luc2 (Photinus pyralis) and Renilla hRluc (Renilla reniformis) genes, which have been codon optimized for more efficient expression in mammalian cells; Both the reporter genes and the vector backbone, including the bla (β-lactamase or Amp r) and mammalian selectable marker genes for hygromycin … chemistry 8th edition changhttp://www.paineltopflash.tk/ chemistry 8th edition robinsonWebRenilla Luciferase Assay Buffer 2.0 quenches the firefly luciferase activity to the level of untransfected cells, allowing sequential measurement of firefly and Renilla luciferase activity in the same sample. This is a flash-type assay that requires luminescence to be measured immediately after adding the detection reagents to the luciferase ... chemistry 8 panelWeb2. apr 2015 · As the renilla here is used as a control for your transfection, which means if your protein of interest is affecting cell proliferation, or inducing any other changes, that … chemistry 8th edition pdfWebThe protocol I follow is as follows: Day 1 : See 0.2e6 HEK293 Top Flash cells (stable cell lines with Renilla/Luc reporters) in 250uL DMEM-10% FBS, on a 24-well plate. Day 2 : Change media with 0 ... chemistry9167 429WebBioAssay record AID 1265312 submitted by ChEMBL: Inhibition of TNSK1/2-activated Wnt pathway in human DLD-1 TOPFlash/EF1a Renilla reporter cells after 24 hrs by Steady-Glo Luciferase assay. chemistry 9701 data booklet 2021