WebDec 15, 1991 · CHIP28 is a 28-kDa integral membrane protein with similarities to membrane channels and is found in erythrocytes and renal tubules. A cDNA for CHIP28 was isolated from human fetal liver cDNA template by a three-step polymerase chain reaction (PCR) cloning strategy, starting with degenerate oligonucleotide primers corresponding to the N … Webidentification of this protein, so-called “CHIP28,” as a water channel has led to the discovery of a large family of water channels and related proteins (reviewed in Ref. 7). This family was first named the MIP family (MIP proteins) as an acronym for the first cloned protein of this family, the major
(PDF) Localization of the CHIP28 water channel in rat …
WebCHIP28) encodes a single tandem repeat of two CHIP28 subunits. Tu prepare cDNAS encoding CHIP28 trimers (and then tetramers), the procedure was repeated using the digested PCR fragment and NcoI- ... The abbreviations used are: PCR, polymerase chain reaction; PBS, phosphate-buffered saline; WT, wild-type; RRL, rabbit reticulocyte ly- WebThe CHIP28 protein is an abundant integral membrane protein in mammalian RBCs and renal proximal tubules and belongs to a family of membrane proteins with unknown functions. Oocytes from Xenopus laevis microinjected with in vitro-transcribed CHIP28 RNA exhibited increased osmotic water permeability; this was reversibly inhibited by mercuric ... high school host club honey
Biogenesis and Transmembrane Topology of the CHIP28 …
WebQuestion: How did the difference in oocytes placed in a hypotonic solution that had the CHIP28 mRNA injected compared to the control oocytes that were not injected indicate to Agre and colleagues that CHIP28 was related to the diffusion of water? Injected oocytes became crenated while control oocytes were unaffected indicating CHIP28 reversed the … WebJan 1, 1993 · CHIP28 is an integral membrane protein that has been identified as the erythrocyte water channel and that is also expressed in the kidney. Antibodies against erythrocyte CHIP28 were used to ... WebJan 5, 1993 · These studies demonstrated: (i) CHIP28 water channel activity is retained despite substitution of individual cysteines with serine; (ii) cysteine 189 is the Hg (2+)-sensitive residue; (iii) the subunits of the CHIP28 complex are individually active water pores; (iv) residue 189 is critical to proper processing of the CHIP28 protein. high school host club characters